منابع مشابه
The effect of minus ends on the microtubule steady state
Dynamic instability describes microtubule assembly in which individual microtubules exhibit alternating phases of elongation and rapid shortening. This dynamic is significantly different for the plus ends and minus ends of a microtubule. In this work, by considering the contribution of the plus and minus ends, we have investigated the behavior of T-tubulin concentration in the steady state in a...
متن کاملPatronin Regulates the Microtubule Network by Protecting Microtubule Minus Ends
Tubulin assembles into microtubule polymers that have distinct plus and minus ends. Most microtubule plus ends in living cells are dynamic; the transitions between growth and shrinkage are regulated by assembly-promoting and destabilizing proteins. In contrast, minus ends are generally not dynamic, suggesting their stabilization by some unknown protein. Here, we have identified Patronin (also k...
متن کاملThe Arabidopsis SPIRAL2 Protein Targets and Stabilizes Microtubule Minus Ends.
The contribution of microtubule tip dynamics to the assembly and function of plant microtubule arrays remains poorly understood. Here, we report that the Arabidopsis SPIRAL2 (SPR2) protein modulates the dynamics of the acentrosomal cortical microtubule plus and minus ends in an opposing manner. Live imaging of a functional SPR2-mRuby fusion protein revealed that SPR2 shows both microtubule plus...
متن کاملForce on spindle microtubule minus ends moves chromosomes
The spindle is a dynamic self-assembling machine that coordinates mitosis. The spindle's function depends on its ability to organize microtubules into poles and maintain pole structure despite mechanical challenges and component turnover. Although we know that dynein and NuMA mediate pole formation, our understanding of the forces dynamically maintaining poles is limited: we do not know where a...
متن کاملNuMA recruits dynein activity to microtubule minus-ends at mitosis
To build the spindle at mitosis, motors exert spatially regulated forces on microtubules. We know that dynein pulls on mammalian spindle microtubule minus-ends, and this localized activity at ends is predicted to allow dynein to cluster microtubules into poles. How dynein becomes enriched at minus-ends is not known. Here, we use quantitative imaging and laser ablation to show that NuMA targets ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2005
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1707iti5